Cilmi-baadhayaashu waxay adeegsadeen dhuumaha cusub ee isotope-ka-cusbooneysii (HT-SIP) iyo metagenomics si ay u helaan muuqaalka ugu horreeya ee microbiome-ka firfircoon ee ku hareeraysan symbiont warshad faa'iido leh, fangaska mycorrhizal arbuscular (AMF). Credit: Lawrence Livermore Shaybaadhka Qaranka
Ku xidhida aqoonsiga microbes duurjoogta ah sifooyinkooda jireed iyo hawlaha deegaanka waa ujeedo fure u ah microbiologists deegaanka. Farsamooyinka ku dadaalaya yoolkan, Stable Isotope Probing-SIP-waxaa loo tixgeliyaa inay tahay tan ugu waxtarka badan ee lagu baranayo noolaha firfircoon ee goobaha dabiiciga ah.
Lawrence Livermore Shaybaadhka Qaranka (LLNL) saynisyahano ayaa soo saaray farsamo cusub —-sare-soo saari SIP — in automates dhowr tallaabo ee habka of baarista isotope deggan, jidaynayey baaritaannada dhaqdhaqaaqa microbial ee microorganisms hoos xaaladaha dhabta ah, iyada oo aan baahida loo qabo dhaqanka shaybaarka.
Gudaha SIP, microbes-ka firfircoon ayaa lagu aqoonsadaa iyada oo la isku daro isotopes deggan oo lagu daro bayomasskooda. Waxay ka mid tahay hababka ugu awoodda badan ee ecology microbial tan iyo markii ay aqoonsan karto microbes firfircoon iyo sifooyinkooda jireed (isticmaalka substrate-ka, biochemistry-ka gacanta, dheef-shiid kiimikaadka, koritaanka, dhimashada) ee bulshooyinka adag ee hoos yimaada xaaladaha hooyo.
Caadi ahaan, habka SIP waxa uu u baahan yahay gacan-ku-qabsasho la taaban karo oo kaliya oo u oggolaanaya tiro yar oo muunado ah. Laakiin farsamada cusub ee LLNL waxay u baahan tahay lix-meelood meel cadadka shaqada gacanta lagu qabto marka la barbar dhigo buug-gacmeedka SIP oo u ogolaanaya 16 muunado in isku mar la farsameeyo.
"Habkeena semi-automated-ka ah wuxuu yareeyaa wakhtiga hawlwadeenka wuxuuna wanaajiyaa taranka iyadoo la beegsanayo tillaabooyinka ugu shaqada badan ee SIP," ayuu yiri saynisyahanka LLNL Erin Nuccio, iyo qoraaga hormuudka ah ee warqad ka soo baxday joornaalka Microbiome. "Waxaan hadda u isticmaalnay habkan si aan uga baaraandegno in ka badan kun muunado, oo ay ku jiraan qaar ka mid ah kuwa aad u yar ee ciidda yar-yar."
Mid ka mid ah microhabitat noocan oo kale ah waa ciidda isla markiiba ku wareegsan unugyada mycorrhizae-nooc ka mid ah fangaska oo sameeya xiriiryo asymbiotic ah oo leh 72% dhammaan dhirta dhulka. Beddelka kaarboonka dhirta, fungus ( fungi mycorrhizal arbuscular) ayaa siiya dadka martida u ah kheyraadka muhiimka ah sida nitrogen, fosfooraska iyo biyaha.
Daraasaddan caddaynta ah ee fikradda, qorayaashu waxay muujiyeen "mareegta cuntada" ee isdhexgalka uu kiciyay fangaska mycorrhizal ee ciidda.
"Waxaan u maleyneynaa in tani ay tahay waddo weyn sida kaarboonka dhirta uu si ballaaran ugu qaybiyo ciidda. Ciiddu waxay haysaa barkadda ugu weyn ee si firfircoon ugu wadida kaarboonka organic ee meeraha, ayay tidhi qoraaga la xidhiidha Jennifer Pett-Ridge, oo ah hogaamiyaha mashruuca LLNL iyo madaxa Waaxda Tamarta ee Xafiiska Sayniska "Microbes-ku adkaysta" Ciidda Microbiome Focus Focus. . "Waxa aanu taxnay qadar yar oo DNA ah, waxaanu go'aansanay noolaha firfircoon ka dibna dib-u-dhiska genome-yadooda iyo isdhexgalka suurtagalka ah."
Qorayaasha kale ee LLNL waxaa ka mid ah Steven Blazewicz, Marissa Lafler, Ashley Campbell, Jeffrey Kimbrel, Jessica Wollard, Rachel Hestrin iyo sidoo kale cilmi baarayaal ka socda Lawrence Berkeley Shaybaarka Qaranka, Machadka Wadajirka Genome ee DOE iyo Jaamacadda California, Berkeley.